Wavelength range: 190-950 nm
- Waters ACQUITY UPLC I-Class PLUS System
- Q-Orbitrap Exploris 240
- Metabolomics services
- High-Performance Liquid Chromatography (HPLC)
- Autoflex speed™ MALDI TOF/TOF
- Q-Exactive/Q-Exactive Plus
- LTQ Orbitrap XL ETD with nano LC-2D
- Gas Chromatography/Mass Spectrometry (GC/MS)
Waters ACQUITY UPLC I-Class PLUS System
High-precision binary pump with an adaptable sample manager for vials and plates and an extensive injection volume range. The autosampler has a refrigerated chamber and high-precision needle for stable, high-throughput workflows.
Location: Building No. 51, Room No. 230 /232
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Q-Orbitrap Exploris 240
The next generation of high-resolution mass-spectrometers that can be used for Data Independent Analysis (DIA) and Parallel Reaction Monitoring (PRM) with a higher scan speed and faster polarity switch. The MS is equipped with an ESI source that provides soft ionization of the molecules. Resolving power is up to 240,000 Full Width at Half Maximum (FWHM). Mass Range for Full Scans is m/z 40–3000 (Scan range: last mass ≤ 15 × first mass), and mass accuracy is 1ppm.
Location: Building No. 51, Room No. 230
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Metabolomics services
The Unit services include:
• Consultation in experiment design
• Assistance with sample preparation method development
• UPLC-MS method development and optimization
• Data acquisition
• Basic and advanced data analysis
• Down-stream support in preparation to publications
• Training
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High-Performance Liquid Chromatography (HPLC)
High-Performance Liquid Chromatography (HPLC) is used for the qualitative and quantitative analysis of organic molecules and organic materials that are not sufficiently volatile for GC analysis (including high–molecular-weight molecules). Laboratory`s HPLC Agilent 1100 Series is equipped with photodiode array and fluorescence detectors.
Location: Building No. 51, Room No. 329
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Autoflex speed™ MALDI TOF/TOF
Matrix-Assisted Laser Desorption/Ionization(MALDI) stands as one of the most powerful
techniques invented for investigating:
1. Biopolymers (such as peptides, proteins, glycansand oligonucleotides)
2. Synthetic polymers and organic molecules (>500 Da), especially in mixtures and crude samples
3. MALDI Imaging. Distribution of proteins,lipid, smallmolecules and etc. in tissue sections.
Results are shown as color coded intensity plots and can be overlaid to an image of the
investigated sample
5. TLC MALDI. Measurements directly from a thin layerchromatography (TLC)
6. Microorganism and Fungi identification and classification
7. Detailed sample characterization by MS/MS.
8.Top down proteomics.
Location: Building No. 51, Room No. 232
Enhanced Speed with a 2 kHz (kiloHertz) proprietary smartbeam-II solid-state laser
(modified Nd:YAG laser) 355 nm and electronics for high throughput applications
including MALDI Imaging, and high-throughput microbiology ID
• Laser focus diameters down to 10 µm for high spatial resolution imaging without
pixel overlap.
• TOF-analyzer for linear and reflectron mode, MS/MS capability TOF-analyzer for
both positive and negative ion mode
• Reflectron resolution ≥ 26000 FWHM ( Somatostatin 28; m/z 3147.47 Da), mass
accuracy < 10ppm (external calibration)
• Linear resolution ≥1,100 for Cytochrome C (m/z 12,361) and ≥ 600 for Protein
A (m/z 44,613) . linear mass accuracy for Cytochrome C <100 ppm (external
calibration)
• Mass accuracy (protein mixture): with external calibration: better than 100 ppm
with internal calibration: better than 90 ppm
• MS sensitivity: 500 fmol BSA (m/z 66,000). S/N ≥ 100
• Expanded mass range capabilities to handle intact proteins and molecules such
as large protein and polymers.
LIFT™ – the latest TOF/TOF technology to enable the use of various MS/MS
techniques, including LID and high-energy CID
• Minimized maintenance with IR laser-based self-cleaning MALDI Perpetual™ Ion
Source, which improves system up-time and utility.
• Optimized Software packages for numerous applications e.g. Protein-, Peptide-,
Polymer-Analyses, and MALDI Imaging, clinical research methods and the
MALDI Biotyper workflow.
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Q-Exactive/Q-Exactive Plus
High-resolution mass-spectrometers that can be used for both Data Independent Analysis (DIA) and Parallel Reaction monitoring (PRM). The MS is equipped with an ESI source that provides soft ionization of the molecules. Resolving power is up to 140,000 Full Width at Half Maximum (FWHM). Mass Range for Full Scans is m/z 50–6000 (Scan range: last mass ≤ 15 × first mass), and mass accuracy is 3ppm.
Location: Building No. 51, Room No. 230
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LTQ Orbitrap XL ETD with nano LC-2D
The LTQ-Orbitrap XL ETD (Thermo Fisher Scientific) is a hybrid Fourier Transform (FT)
mass spectrometer with high sensitivity, high mass accuracy, speed and capable of MSn.
It can make concurrent high resolution (up to 100,000) and accurate mass measurements
(≤ 2 ppm) of molecules.
It is the most comprehensive solution for complex post translation analysis, intelligent sequencing
of peptides, top-down,middle-down proteomics and analysis of organic molecules
Location: Building No. 51, Room No. 230
Resolution
60000(WHM) @ m/z 400 with a scan repetition rate of 1 second.
Minimum resolution 7500 & maximum resolution 100000 @ m/z 400.
Cycle Time
scan at 60000 resolution @ m/z 400 per second.
Mass Range
m/z 50–2000 ; m/z 200–4000.
Mass Accuracy
≤ 3ppm RMS for 2h period with external calibration using defined conditions.
≤ 2ppm RMS with internal calibration.
Dynamic Range
≥ 10000 between mass spectra & >4000 between highest and lowest detectable ion signal in
one spectrum.
MS/MS
MS/MS and MSn scan functions.
Nano LC – 2D specifications
Ultra-low flow rates of first dimension 1-20 µL/min, second dimension 50-1000 nL/min.
Pumping system eliminates the need for flow splitting, providing excellent run-to-run reproducibility.
Active flow control of each mobile phase ensures precise gradient delivery.
Quickly responds to changes in flow rate set point in less than two seconds.
Reduce solvent consumption by 99%.
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Gas Chromatography/Mass Spectrometry (GC/MS)
Gas Chromatography/Mass Spectrometry (GC/MS) is applied for the separation and identification of a variety of organic compounds. The Unit’s Agilent 7890N GC is connected to an Agilent 5977A single-quadrupole mass-selective detector. The instrument is equipped with a 100-vial autosampler, an NIST02 MS Library (includes 175,000 spectra for 147,000 compounds) and an ACD Labs MS Manager (software package for mass-spectra interpretation and structure elucidation).
Location: Building No. 51, Room No. 329
Performance electronics for 12,500 u/s scan speed (8,000 u/s write-to-disk)
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