- Image analysis services
- Support for processing and analyzing fluorescence microscopy images
- Implementation and integration of tools for obtaining quantitative information from micrographs
- Consultation for optimizing output in microscopy, and training of researchers on the use of bioimage analysis tools
- Zeiss LSM880 Airyscan
- Fv1000 laser scanning confocal
- ImageStreamX mkII
- FACSAria III
- SY3200 cell sorter
- SP6800 spectral analyzer
- EC800 cell analyzer
- Spinning Disk
- ProteOn XPR36
- Carboxyl coated chips (for binding proteins via an amine group)
- Neutravidin coated chips (for binding a biotinylated molecule)
- Tris-NTA coated chips (for binding his tagged proteins)
The Image Analysis service offers:
Confocal microscope with super resolution (140nm) and spectral imaging
6 excitation lasers (405nm, 458bm, 488nm, 514nm, 561nm, 633nm). 2 PMT detectors, 32 Channels GaAsp spectral detector, Airyscan imaging. fully incubated for live cell imaging. Wide selection of objectives for different application. 2 excitation lasers (488nm, 642nm).
405, 488, 561 and 640nm excitation lasers
High throughput imaging of cells and particales in liquid suspension. By combining the speed, sensitivity, and phenotyping abilities of flow cytometry with the detailed imagery and functional insight of microscopy, the ImageStreamX Mark II overcomes the limitations of both techniques and opens the door to an extensive range of novel applications.
4 Fluorescent channels, 20x 40x 60 objectives. IDEAS data analysis software.
Fluorescence-Activated Cell Sorting (FACS) is a method for analysis and sorting of cells and other biological particles in liquid suspension(e.g. exosomes) based on light scattering and fluorescence characterizations.
4 excitation lasers (405nm, 488nm, 561nm, 635nm). 13 Fluorescent channels. up to 4 populations sorting, Single cell sorting
The SY3200 can analyze\sort cells based on their Size, density, expression of fluorescent proteins and the ability to bind fluorescent Ab’s
2 sorting modules:
HAPS1: 375nm, 488nm, 561nm and 590nmc excitation lasers
HAPS2: 405, 488 and 640nm excitation lasers.
13 Fluorescent channels. up to 4 populations sorting, Single cell sorting
Located in a biological hood for sterile sorting conditions.
The SP6800 is specially adapted to analyze multi stain fluoresenctly labelled cells without the need for complicated compensation corrections
405, 488 and 640 nm excitation lasers
The EC800 can analyze cells based on their Size, density, expression of fluorescent proteins and the ability to bind fluorescent Ab’s
Has a 488 and 640nm lasers.
Contains an auto loader unit for tubes and plates.
Confocal imaging via spinning disk involves scanning a field with laser light from a number of pinholes arranged in a pattern on a modified Nipkow disk. Unlike laser scanning confocal microscopes (LSM) which scan one point of laser light across an entire field, a spinning disk confocal scans approximately 1,000 points of laser light across the field simultaneously resulting in much faster image production. In a traditional LSM, the detector is a photomultiplier tube which can register the signal from only one point of light (pixel) at a time and with a typical quantum efficiency of 40-50%. In an SDC the detector is a CCD camera which can register the signal from a quarter million or million pixels simultaneously with a quantum efficiency upwards of 95%. The result is that while LSMs can typically image on the order of one full frame per second, SDCs can image at over 1,000 frames per second. This significant speed difference combined with the superior sensitivity of high-end CCDs has made spinning disk confocal a must-have technology for advanced live cell imaging labs.
Measures affinity and kinetic parameters between molecules in high throughput mode
6 channels for ligand binding and 6 channels for analyte bindings
Various chips (for ligand binding) can be purchased from the manufacture (Xantec):
Other chips are also available
All chips can be bought with various binding site densities to optimize detection of analyte binding to the ligand.