Zeiss Celldiscoverer 7 (CD7) is a widefield based platform that combines several hardware and software element to allow long lasting live cell fluorescent and brightfield imaging. The system can accommodate wide verity of sample carriers (glass or plastic) and can be easily used with multi-well plate. CD7 has unique adjustable optical setup that supports wide range of working distances. In combination with Zen image analysis tools, CD7 can generate high-quality and high-throughput data on single cell level (“flow cytometry like”) at spatial and temporal dimensions. CD7 is also suitable platform to image large sample such as tissue sections.