Macromolecular Crystallography Research Center
The unit aims to provide a three-dimensional structure of the protein of interest using X-ray Crystallography.
We use a fully automated protein crystallization process provided by the unit, starting from the design of the experiment and finishing with imaging of the crystallization plates under a user-defined schedule. This process saves time and material as it relies on robotics which uses tiny amounts of a sample.
We operate an X-ray generator with an image plate area detector for crystal screening and data collection. We also maintain all the computer hardware and software needed for structure determination and analysis.
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Electron Microscopy Unit
The IKI Electron Microscopy Unit provides expertise and resources for solving macromolecular structures. Our new high-end transmission electron microscope enables high-resolution structure determination of macromolecular complexes. We provide access to Thermo-Fisher Glacios, with the state-of-the-art Falcon 4i direct detection camera coupled to a Selectris X energy filter for high-contrast micrographs. All the required computational support necessary for single-particle analysis is also available for our users. Higher-order structure determination is enabled by tomographic reconstructions using the same setup. Grids sample preparation expertise and equipment (Leica EM GP) are also available.
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Computational Structural Biology
Computational Structural Biology provides expertise in combining experimental and theoretical methods to study the correlation between the 3D structure and dynamics of bio-molecules and their activity and regulation. At the IKI I am working with various researchers to implement computational structural methods – such as modelling, molecular dynamics simulations or computational protein design – into their research.