Bio-imaging and Flow Cytometry

The Zeiss Elyra 7 is a state-of-the-art super-resolution imaging platform available at our facility, offering exceptional flexibility for both live-cell and fixed-sample imaging. It combines multiple advanced imaging modalities—including Lattice SIM², Single Molecule Localization Microscopy (SMLM), and Apotome optical sectioning—to provide nanoscale resolution, fast acquisition speeds, and compatibility with a wide range of biological samples.
________________________________________

Lattice SIM²: Fast, Gentle, and Super-Resolved
Lattice SIM² is an advanced structured illumination method that enables super-resolution imaging down to ~60 nm while maintaining low phototoxicity—making it ideal for live-cell experiments. It uses a lattice pattern of light to extract high-resolution information from standard fluorophores and produces fast, high-contrast 3D images with minimal photodamage.
To further enhance performance:
• Leap mode enables rapid volumetric imaging with improved axial resolution, making it ideal for capturing fast 3D biological processes.
• Burst mode captures sequences at extremely high speed to reduce motion blur and allow real-time observation of rapid cellular events.
________________________________________

SMLM (PALM/STORM): Nanoscale Precision, Molecule by Molecule
Single Molecule Localization Microscopy (SMLM) techniques, such as PALM and STORM, push resolution down to 20 nm or better by localizing individual fluorophores with extreme precision. This mode is best suited for fixed samples where fine molecular details—such as protein clusters, membrane domains, or cytoskeletal structures—need to be resolved.
________________________________________

Apotome: Enhanced Widefield Imaging
The Elyra 7 also includes Apotome, a structured illumination technique for widefield microscopy that removes out-of-focus light to produce optically sectioned images. It’s a fast and simple way to improve contrast and resolution in thicker specimens, especially for routine fluorescence imaging.
________________________________________

With these advanced imaging capabilities, the Elyra 7 system supports a broad spectrum of applications—from live-cell dynamics and 3D organelle tracking to ultra-high-resolution structural mapping of fixed samples. Contact us to learn more, schedule training, or reserve time on the system.

Zeiss Celldiscoverer 7 (CD7) is a widefield based platform that combines several hardware and software element to allow long lasting live cell fluorescent and brightfield imaging. The system can accommodate wide verity of sample carriers (glass or plastic) and can be easily used with multi-well plate. CD7 has unique adjustable optical setup that supports wide range of working distances. In combination with Zen image analysis tools, CD7 can generate high-quality and high-throughput data on single cell level (“flow cytometry like”) at spatial and temporal dimensions. CD7 is also suitable platform to image large sample such as tissue sections.

The SY3200 can analyze\sort cells based on their Size, density, expression of fluorescent proteins and the ability to bind fluorescent Ab’s

The SP6800 is specially adapted to analyze multi stain fluoresenctly labelled cells without the need for complicated compensation corrections

The EC800 can analyze cells based on their Size, density, expression of fluorescent proteins and the ability to bind fluorescent Ab’s

Confocal imaging via spinning disk involves scanning a field with laser light from a number of pinholes arranged in a pattern on a modified Nipkow disk. Unlike laser scanning confocal microscopes (LSM) which scan one point of laser light across an entire field, a spinning disk confocal scans approximately 1,000 points of laser light across the field simultaneously resulting in much faster image production. In a traditional LSM, the detector is a photomultiplier tube which can register the signal from only one point of light (pixel) at a time and with a typical quantum efficiency of 40-50%. In an SDC the detector is a CCD camera which can register the signal from a quarter million or million pixels simultaneously with a quantum efficiency upwards of 95%. The result is that while LSMs can typically image on the order of one full frame per second, SDCs can image at over 1,000 frames per second. This significant speed difference combined with the superior sensitivity of high-end CCDs has made spinning disk confocal a must-have technology for advanced live cell imaging labs.

Measures affinity and kinetic parameters between molecules in high throughput mode