Please see description of available software tools at the technical specification sheet .
- Zeiss Celldiscoverer 7 – Automated live/fixed fluorescence imaging
- Image analysis services
- Support for processing and analyzing fluorescence microscopy images
- Implementation and integration of tools for obtaining quantitative information from micrographs
- Consultation for optimizing output in microscopy, and training of researchers on the use of bioimage analysis tools
- Zeiss LSM880 Airyscan
- Fv1000 laser scanning confocal
- ImageStreamX mkII
- FACSAria III
- SY3200 cell sorter
- SP6800 spectral analyzer
- EC800 cell analyzer
- Spinning Disk
- ProteOn XPR36
- Carboxyl coated chips (for binding proteins via an amine group)
- Neutravidin coated chips (for binding a biotinylated molecule)
- Tris-NTA coated chips (for binding his tagged proteins)
Zeiss Celldiscoverer 7 – Automated live/fixed fluorescence imaging
Zeiss Celldiscoverer 7 (CD7) is a widefield based platform that combines several hardware and software element to allow long lasting live cell fluorescent and brightfield imaging. The system can accommodate wide verity of sample carriers (glass or plastic) and can be easily used with multi-well plate. CD7 has unique adjustable optical setup that supports wide range of working distances. In combination with Zen image analysis tools, CD7 can generate high-quality and high-throughput data on single cell level (“flow cytometry like”) at spatial and temporal dimensions. CD7 is also suitable platform to image large sample such as tissue sections.
Location : Building No. 51, Room No. 019
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Image analysis services
The Image Analysis service offers:
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Zeiss LSM880 Airyscan
Confocal microscope with super resolution (140nm) and spectral imaging
Location: Building No.51, Room No.326
6 excitation lasers (405nm, 458bm, 488nm, 514nm, 561nm, 633nm). 2 PMT detectors, 32 Channels GaAsp spectral detector, Airyscan imaging. fully incubated for live cell imaging. Wide selection of objectives for different application. 2 excitation lasers (488nm, 642nm).
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Fv1000 laser scanning confocal
Location: Building No.39, Room No.309
405, 488, 561 and 640nm excitation lasers
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ImageStreamX mkII
High throughput imaging of cells and particales in liquid suspension. By combining the speed, sensitivity, and phenotyping abilities of flow cytometry with the detailed imagery and functional insight of microscopy, the ImageStreamX Mark II overcomes the limitations of both techniques and opens the door to an extensive range of novel applications.
Location: Building No.51, Room No.326
4 Fluorescent channels, 20x 40x 60 objectives. IDEAS data analysis software.
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FACSAria III
Fluorescence-Activated Cell Sorting (FACS) is a method for analysis and sorting of cells and other biological particles in liquid suspension(e.g. exosomes) based on light scattering and fluorescence characterizations.
Location: Building No.51, Room No.326
4 excitation lasers (405nm, 488nm, 561nm, 635nm). 13 Fluorescent channels. up to 4 populations sorting, Single cell sorting
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SY3200 cell sorter
The SY3200 can analyze\sort cells based on their Size, density, expression of fluorescent proteins and the ability to bind fluorescent Ab’s
Specification
2 sorting modules:
HAPS1: 375nm, 488nm, 561nm and 590nmc excitation lasers
HAPS2: 405, 488 and 640nm excitation lasers.
13 Fluorescent channels. up to 4 populations sorting, Single cell sorting
Located in a biological hood for sterile sorting conditions.
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SP6800 spectral analyzer
The SP6800 is specially adapted to analyze multi stain fluoresenctly labelled cells without the need for complicated compensation corrections
Location: Building No. 39, Room No.309
405, 488 and 640 nm excitation lasers
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EC800 cell analyzer
The EC800 can analyze cells based on their Size, density, expression of fluorescent proteins and the ability to bind fluorescent Ab’s
Location: Building No. 39, Room No.309
Has a 488 and 640nm lasers.
Contains an auto loader unit for tubes and plates.
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Spinning Disk
Confocal imaging via spinning disk involves scanning a field with laser light from a number of pinholes arranged in a pattern on a modified Nipkow disk. Unlike laser scanning confocal microscopes (LSM) which scan one point of laser light across an entire field, a spinning disk confocal scans approximately 1,000 points of laser light across the field simultaneously resulting in much faster image production. In a traditional LSM, the detector is a photomultiplier tube which can register the signal from only one point of light (pixel) at a time and with a typical quantum efficiency of 40-50%. In an SDC the detector is a CCD camera which can register the signal from a quarter million or million pixels simultaneously with a quantum efficiency upwards of 95%. The result is that while LSMs can typically image on the order of one full frame per second, SDCs can image at over 1,000 frames per second. This significant speed difference combined with the superior sensitivity of high-end CCDs has made spinning disk confocal a must-have technology for advanced live cell imaging labs.
Location: Building No. 51, Room No. 019
For technical specification, please click here
and for more information, click here
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ProteOn XPR36
Measures affinity and kinetic parameters between molecules in high throughput mode
Location: Building No. 39, Room No.309
6 channels for ligand binding and 6 channels for analyte bindings
Various chips (for ligand binding) can be purchased from the manufacture (Xantec):
Other chips are also available
All chips can be bought with various binding site densities to optimize detection of analyte binding to the ligand.
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