The Zeiss Elyra 7 is a state-of-the-art super-resolution imaging platform available at our facility, offering exceptional flexibility for both live-cell and fixed-sample imaging. It combines multiple advanced imaging modalities—including Lattice SIM², Single Molecule Localization Microscopy (SMLM), and Apotome optical sectioning—to provide nanoscale resolution, fast acquisition speeds, and compatibility with a wide range of biological samples.
________________________________________

Lattice SIM²: Fast, Gentle, and Super-Resolved
Lattice SIM² is an advanced structured illumination method that enables super-resolution imaging down to ~60 nm while maintaining low phototoxicity—making it ideal for live-cell experiments. It uses a lattice pattern of light to extract high-resolution information from standard fluorophores and produces fast, high-contrast 3D images with minimal photodamage.
To further enhance performance:
• Leap mode enables rapid volumetric imaging with improved axial resolution, making it ideal for capturing fast 3D biological processes.
• Burst mode captures sequences at extremely high speed to reduce motion blur and allow real-time observation of rapid cellular events.
________________________________________

SMLM (PALM/STORM): Nanoscale Precision, Molecule by Molecule
Single Molecule Localization Microscopy (SMLM) techniques, such as PALM and STORM, push resolution down to 20 nm or better by localizing individual fluorophores with extreme precision. This mode is best suited for fixed samples where fine molecular details—such as protein clusters, membrane domains, or cytoskeletal structures—need to be resolved.
________________________________________

Apotome: Enhanced Widefield Imaging
The Elyra 7 also includes Apotome, a structured illumination technique for widefield microscopy that removes out-of-focus light to produce optically sectioned images. It’s a fast and simple way to improve contrast and resolution in thicker specimens, especially for routine fluorescence imaging.
________________________________________

With these advanced imaging capabilities, the Elyra 7 system supports a broad spectrum of applications—from live-cell dynamics and 3D organelle tracking to ultra-high-resolution structural mapping of fixed samples. Contact us to learn more, schedule training, or reserve time on the system.