LTQ Orbitrap XL ETD with nano LC-2D

The LTQ-Orbitrap XL ETD (Thermo Fisher Scientific) is a hybrid Fourier Transform (FT)
mass spectrometer with high sensitivity, high mass accuracy, speed and capable of MSn.
It can make concurrent high resolution (up to 100,000) and accurate mass measurements
(≤ 2 ppm) of molecules.
It is the most comprehensive solution for complex post translation analysis, intelligent sequencing
of peptides, top-down,middle-down proteomics and analysis of organic molecules

Location: Building No. 51, Room No. 230

Resolution
60000(WHM) @ m/z 400 with a scan repetition rate of 1 second.
Minimum resolution 7500 & maximum resolution 100000 @ m/z 400.
Cycle Time
scan at 60000 resolution @ m/z 400 per second.
Mass Range
m/z 50–2000 ; m/z 200–4000.
Mass Accuracy
≤ 3ppm RMS for 2h period with external calibration using defined conditions.
≤ 2ppm RMS with internal calibration.
Dynamic Range
≥ 10000 between mass spectra & >4000 between highest and lowest detectable ion signal in
one spectrum.
MS/MS
MS/MS and MSn scan functions.
Nano LC – 2D specifications
Ultra-low flow rates of first dimension 1-20 µL/min, second dimension 50-1000 nL/min.
Pumping system eliminates the need for flow splitting, providing excellent run-to-run reproducibility.
Active flow control of each mobile phase ensures precise gradient delivery.
Quickly responds to changes in flow rate set point in less than two seconds.
Reduce solvent consumption by 99%.

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