Cryo EM-GLACIOS

The Thermo Fisher Scientific Glacios is a 200 kV transmission electron microscope with X-FEG and Falcon 4i detector coupled to a Selectris X energy filter for high-resolution structure determination of biological (and other soft matters) macromolecules. Its 12-grid autoloader allows efficient loading and unloading of cryo-EM grids. The automated EPU and Tomo software systems are used for automated data collection, capable of producing >300 movies/hour, for high-resolution single particle analysis and cryo-electron tomography.

Location: Building No. 39, Room No. -118

Accelerating Voltage	200 kV
Electron Source	X-FEG
Stage Tilt Range	± 70°
C2 Apertures	150, 100, 70 and 50 µm
Objective Apertures	100, 70 and 30 µm
Operating Temperature	~80 K
Information Limit at 0° / ±70° tilt	<0.2 nm / 0.34 nm
Cs	2.7 mm
Number of cartridge positions for grids	12 total
Sample exchange time	~3 mins from autoloader cassette to stage
Camera	Falcon 4i
Camera pixels	14×14 micrometers pixel size, 4096×4096 pixels, Electron Event Recording
The Selectris X is designed for stability and atomic resolution imaging Narrow (<10 eV) zero-loss energy filtering offers enhanced contrast for higher-resolution reconstructions Aberration correction, low image distortions, and uniform energy resolution over the entire field of view