The Tecnai T12 G2 TWIN TEM Thermo Fisher Scientific (former FEI) Transmission Electron Microscope (TEM) is an instrument with a design combining high contrast and resolution essential to visualizing image details in a wide range of samples at both room and cryogenic (cryo) temperatures. This microscope enables studies in a broad spectrum of science from hard to soft matter. On one hand, the high tilt capabilities of this microscope, equipped with single tilt holder, allow to perform high quality studies of crystalline materials. On the other hand, the set up in our laboratory, including a Low-Dose exposure technique, is configured to be optimal for cryo-TEM.