The Octet R8e is a BLI (Bio-Layer Interferometry)-based instrument used to measure the binding affinity between two molecules.

Samples are loaded into 96- or 384-well plates. The ligand is immobilized on an optical sensor, allowing up to eight samples to be analyzed simultaneously. The sensor is then incubated with the analyte, and the interaction is monitored in real time to determine the association rate (Ka), dissociation rate (Kd), and equilibrium dissociation constant (KD).

A wide variety of biosensors is available for capturing His-tagged, biotin-tagged, and Fc-tagged proteins. Alternatively, ligands can be immobilized using standard amine coupling chemistry. Specialized biosensors for nanobody capture are also available.