The Monolith NT.115 measures affinity in steady state by using small amounts of samples (ul range) as follows:
• One molecule (that is labeled with a fluorescent blue\green excited dye) is incubated with dilutions of the other molecules.
• The mixed samples are transferred to capillaries and subjected to a temperature gradient.
• Changes in fluorescence (due to heat or migration) are monitored
• A plot of change in fluorescence vs. concentration helps calculate the affinity at steady state.
The whole process can take less than 30 minutes.